What is tissue fixation

Fixation

 

Fixation means killing the tissue as gently as possible while maintaining the cell structure.

 

Fixing a specimen can be necessary for many reasons.

  • Stop movement - maintain a state
  • Fixed specimens show a stronger refraction of light and thus a higher contrast
  • Fixed tissue is more stable - making incisions
  • Some stains are not possible without fixation
  • Preparation of a permanent preparation

 

Methodologically, a distinction can be made between

  • physical fixation: by rapid freezing (cooling from 10,000 ° / sec)
    to avoid ice crystal formation.
  • chemical fixation: Molecules of the cell are made by chemicals
    networked with each other

 


literature

  • Braune W., Leman A., Taubert H .: Plant anatomical practical course II. Introduction to the structure, the reproductive process and the ontogeny of the lower plants and the embryology of the spermatophyta. Gustav Fischer Verlag; Jena; 1982
  • Plattner H., Zingsheim H. P .: Electron microscopic methodology in cell and molecular biology. A critical guide to biological ultrastructural research for biologists and medical professionals. Gustav Fischer Verlag; Stuttgart, New York; 1987