What is tissue fixation



Fixation means killing the tissue as gently as possible while maintaining the cell structure.


Fixing a specimen can be necessary for many reasons.

  • Stop movement - maintain a state
  • Fixed specimens show a stronger refraction of light and thus a higher contrast
  • Fixed tissue is more stable - making incisions
  • Some stains are not possible without fixation
  • Preparation of a permanent preparation


Methodologically, a distinction can be made between

  • physical fixation: by rapid freezing (cooling from 10,000 ° / sec)
    to avoid ice crystal formation.
  • chemical fixation: Molecules of the cell are made by chemicals
    networked with each other



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